Fig. S6.

Dot blot assay for the specificity of the designed antibodies. Dot blot assay for the binding (from left to right) of DesAb-F DesAb-Aβ, DesAb-IAPP, and two-loop DesAb to same concentrations (x axis) of purified αSyn (green bars, top three lines of dots), synthetic Aβ42 (yellow bars, middle three lines of dots), and IAPP (blue bars, lower three lines of dots) mixed to an E. coli lysate. The bar plot is a quantification of the intensities of the DesAb dot blots (SI Materials and Methods). Intensities are *>2 ${\sigma }_{eq}$ away, ** > 3 ${\sigma }_{eq}$, and *** > 4 ${\sigma }_{eq}$ with ${\sigma }_{eq}=\sqrt{S{E}_{+}^2+S{E}_{-}^2}$, SE being the standard error from the intensities of the three dots.