Fig. 5.

Overexpression of U2AF⁶⁵ inhibits pre-mRNA splicing of Ad ML and β-globin. (A, Upper) The schemes of chimeric minigenes are shown. Introns from SMN pre-mRNAs are shown with thicker lines; introns from Ad ML pre-mRNAs are shown with thinner lines. A₁ and A₂ indicate exons from Ad ML pre-mRNA. (Lower) Analysis of intron splicing in different minigenes are shown. (B) (Left) Intron splicing of Ad ML pre-mRNA with a different amount (µg) of U2AF⁶⁵ plasmid. (Right) Intron splicing of Ad ML pre-mRNA at 2-, 4-, and 6-h points with U2AF⁶⁵ expression. (C, Upper) Scheme of SMN1/2-E7 plasmids. (Lower) RT-PCR analysis of RNAs extracted from 293A cells that expressed SMN1/2 E7 and Flag-U2AF⁶⁵ or control plasmid. (D, Upper) Scheme of β-globin pre-mRNA. (Lower) Intron splicing of β-globin under U2AF⁶⁵ expression is shown.