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Both RS domain and RRM domain are required for the role of U2AF65 in exon 7 skipping of SMN1 pre-mRNA. RT-PCR analysis of SMN1 minigene in 293A cells that overexpressed Flag-U2AF65 (or Flag-U2AF65 ΔRS or Flag-U2AF65 RS) plasmid or control plasmid are shown.
