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. 2015 Jul 27;112(32):E4485–E4494. doi: 10.1073/pnas.1503766112

Fig. 1.

Fig. 1.

Two-photon excitation of CYAM. (A) Multiphoton excitation spectrum of 2 mM CYAM in phosphate-citrate buffer at pH 5.0 (●), pH 6.0 (▪), and pH 7.0 (▲). CYAM fluorescence was measured and background-subtracted at the indicated wavelengths and pH values. Data are not corrected for changes in laser output with wavelength. (B) Two-photon excitation of CYAM. CYAM (2 mM) in phosphate-citrate buffer at pH 5 was excited with increasing levels of power at 780 nm. Circles (●) indicate individual data points, and the line represents the linear regression through those points with a slope of 2.04. AU, arbitrary unit; F, fluorescence.