Fig. 9.

VMAT does not affect CYAM accumulation, but mediates depletion. (A) CYAM accumulation is VMAT-independent. Striatum slices were pretreated with aCSF followed by CYAM treatment (control) or pretreated for 30 min with 500 nM reserpine followed by 1 h of coincubation with CYAM. (Bottom) Quantification of CYAM accumulation of six, 60 × 60-μm images per treatment is displayed as mean ± SEM. (B) MPP⁺, a positively charged VMAT substrate, mimics pCA-induced changes in CYAM fluorescence. 2P image of CYAM-treated striatum slice treated with 50 μM MPP⁺ for 20 min. (Bottom) Quantification of the change in CYAM fluorescence with MPP⁺ treatment (n = 4–7 slices per treatment). (Scale bars: 2 μm.) *P < 0.05.