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DNP-enhanced 15N cross-polarization (CP) spectrum (CP + DNP) and double cross-polarization (DCP) –filtered 15N spectrum (DCP + DNP) of ChR2. (Inset) Comparison of the correlation of the absorption maximum with the 15N Schiff base chemical shift for ChR2, green proteorhodopsin (GPR), bacteriorhodopsin (BR), and model compounds. Modified from ref. 54.
