Fig. 7.

FraG N-terminal localization. (A) Cartoon showing the different domains of FraG and the location of the GFP insertion in the linker domain of FraG in the pRGF plasmid. The plasmid was introduced into both WT and ∆fraG yielding WGF and ∆GF. (B and C) Autofluorescence (red) and GFP fluorescence (green) in WGF grown in N+ and N−, respectively. (D) Autofluorescence (red) and GFP fluorescence (green) in W30 grown in N+ (Note that the mutant cannot grow in N− media). (E) Same micrograph shown in D rotated 45° around the y axis and showing only GFP fluorescence. Notched arrowheads indicate the location of the CCL-GFP construct in the divisome plane. Straight arrowheads indicate cells at the end of division, hence the presence of a GFP signal. GFP fluorescence shows the FraG N-terminal-linker domain as rings in the divisome plane of vegetative cells. CC, predicted Coiled Coil; C ter, C-terminal domain; L, predicted Linker domain; M1, FraG first Methionine; N-ter, N-terminal domain; P391, Proline- the 391st amino acid in FraG linker domain where GFP was fused; TM, Transmembrane domain. (Scale bar: 2 μm.)