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. 2015 Jul 6;112(32):10014–10019. doi: 10.1073/pnas.1507534112

Fig. S1.

Fig. S1.

(A) IB detecting proteasome components Poh1, 20S alpha subunits 1–7, Rpn13, or β-Actin (loading control) in TE671 expressing shScr or Poh1-specific shRNA (shPoh1). (B and C) MEFs transfected with nonspecific siRNA (siControl) or Poh1-specific siRNA (siPoh1). Quantitative RT-PCR performed and Poh1 (B) or CXCL10 (C) mRNA relative to TBP mRNA copies quantified by ∆∆Ct method (D) IB detecting Poh1 or β-Actin (loading control) in 293T transfection with siControl or siPoh1. (E) 293T transfected with siControl or siPoh1 were subsequently transfected with NF-κB-luciferase and TK-renilla luciferase and increasing amount of IKKβ and luciferase values read 24 h posttransfection. In parallel, 293T were treated with 50 nM bortezomib or DMSO vehicle for the duration of the IKKβ transfection. Firefly luciferase values normalized relative to renilla luciferase values and expressed relative to empty vector control.