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. 2015 Jul 6;112(32):10044–10049. doi: 10.1073/pnas.1511570112

Fig. 3.

Fig. 3.

Isolated chloroplasts form stromules. N. benthamiana chloroplasts labeled with stromal GFP (A, green) or CFDA staining (B, yellow) show stromules after isolation from their cellular environment. (C) Chloroplasts isolated from S. oleracea and stained with CFDA (yellow; chlorophyll autofluorescence, red) also have stromules. (D and E) Isolated N. benthamiana chloroplasts labeled with stromal GFP form new stromules over time. Newly forming stromules are indicated by white arrows. (F and G) N. benthamiana chloroplasts isolated with a Percoll purification step and labeled with stromal GFP (green; chlorophyll autofluorescence, red) also have stromules.