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Representative images of stromule frequency over the course of 48 h during a 12-h light/12-h dark cycle. N. benthamiana chloroplast stromule frequency is high throughout the day (A, dawn; C, 4 h after dawn; E, 8 h after dawn) and low throughout the night (B, dusk; D, 4 h after dusk; F, 8 h after dusk). Chloroplasts and stromules are labeled with stromal GFP. Some stromules are indicated by white arrows. (Scale bars: 10 μm.)
