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Identification of GUN2 orthologs in N. benthamiana. A gene tree of heme oxygenases arranged homologs into two clades: HO1 (or GUN2)-like and HO2-like. (Upper) N. benthamiana has two HO1 homeologs. (Lower Left) The silencing trigger against NbGUN2 was designed to cover the entire gene sequence (837 bp). (Lower Right) Silencing NbGUN2 caused expected phenotypes, such as chlorosis.
