Fig. S5.

Adult Srf^iECKO animals display common and differential pathological characteristics at initial vs. acute stages. Adult control (Left), initial-stage Srf^iECKO (Center), and acute-stage Srf^iECKO (Right) animals are compared regarding disease-associated processes. H&E staining reveals brain hemorrhages in initial-and acute-stage Srf^iECKO animals, with erythrocytes being localized in the surrounding tissue outside blood vessels. PAS reactivity (arrowheads) shows plasma extravasation in both Srf^iECKO disease stages. Turnbull Blue staining, indicating aged iron deposits, is seen in acute-stage Srf^iECKO brains only. Kongo Red, a marker for amyloid plaques, which occur in cerebral amyloid angiopathy patients, is negative in control and both Srf^iECKO stages. Nissl staining indicates neuronal degeneration only in the acute-stage Srf^iECKO animals (arrow). (Scale bars, 50 and 100 μm, as indicated in the images.)