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. 2015 Jul 27;112(32):9990–9995. doi: 10.1073/pnas.1510837112

Fig. S2.

Fig. S2.

Construction and confirmation of Beas/2b-19del and Beas/2b-21L858R cells. Beas/2b-19del and Beas/2b-21L858R cell lines were constructed by transfection with the indicated plasmids (pCAG-IN-EGFR-19del -P2A-EGFP and pCAG-IN-EGFR-L858R-P2A-EGFP). (A) Both Beas/2b-19del and Beas/2b-21L858R cells could form foci in soft agar that were significantly larger than those of Beas/2b cells. These two cell lines could also form tumors in nude mice, whereas the Beas/2b cell line could not. (B and C) Balb/C nude mice were s.c. engrafted with Beas/2b-19del and Beas/2b-21L858R cells, and tumors were treated daily with vehicle control or erlotinib at the indicated dose. The tumor volumes (y axis) are plotted over time (x axis). B and C were replotted using data of Fig. 4C to show growth of xenografts following erlotinib treatment.