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. 2015 Aug 15;25(5):634–650. doi: 10.1111/bpa.12281

Figure 4.

figure

H5N1  virus infection of the ferret. A,B. The histopathology of the lung from a ferret infected with H5N1 virus 5 days previously. A. Hematoxylin & eosin (H&E)‐stained paraffin section demonstrates a severe broncho‐ and alveolar pneumonia. B. Differential interference contrast and in situ hybridization for influenza matrix protein RNA (black grains) demonstrates infected alveolar cells in lower airway at 2 days post‐infection (DPI). C. Whole mount of the ferret brain 6 DPI with H5N1 virus hybridized with radioactive probes to influenza matrix protein RNA (black grains) demonstrates multifocal infection in olfactory cortex, cerebral cortex, deep gray nuclei and brainstem. D. In situ hybridization for influenza matrix protein RNA (black grains) (counterstained with hematoxylin) shows infected cells in liver surrounding intense inflammatory nodules. E. In situ hybridization for influenza matrix protein RNA (black grains) (counterstained with hematoxylin) illustrates infected cells in splenic red pulp at 18 DPI. F. Double label in situ hybridization for influenza matrix protein RNA (red) and immunohistochemistry for neurofilament (green) shows infection of neuronal elements.