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. Author manuscript; available in PMC: 2016 Feb 14.
Published in final edited form as: Nat Commun. 2015 Aug 14;6:8005. doi: 10.1038/ncomms9005

Figure 2.

Figure 2

Protein expression levels of HDAC4 Full Length and HDAC4 deletion alleles. (a) Assay of HDAC4 expression using anti-HDAC4 immunohistochemistry in the electroporated rd1 retinas at P12. Scale bar, 40 μm. (b) The intensity of anti-HDAC4 immunofluorescence was quantified as a ratio to the fluorescence intensity of co-expressed GFP. *P<0.01. Data are presented as mean ± s.d., n=3. (c) Assay of HDAC4 expression at P12 using anti-HDAC4 western blots in the electroporated rd1 retinas, and quantified (d) after normalization to the co-expressed GFP. *P<0.01. Data are presented as mean ± s.d., n=3. (e) Quantitative PCR analysis of mRNA levels of HDAC4 alleles at P12 in the electroporated rd1 retinas. n=3.