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. Author manuscript; available in PMC: 2016 Aug 13.
Published in final edited form as: Cell. 2015 Aug 13;162(4):780–794. doi: 10.1016/j.cell.2015.07.013

Figure 3. Wnt3a Activates YAP/TAZ Independent of LRP5/6.

Figure 3

(A) Wnt3a activates YAP/TAZ in LRP5/6 DKO cells. LRP5/6 DKO MEFs were starved for 16 hr then stimulated with Wnt3a (100 ng/ml). LRP5/6 deletion was confirmed by western blot.

(B) LRP5/6 are required for Wnt3a–induced expression of β-catenin target gene Axin2, but not YAP/TAZ target gene Ctgf. Wnt3a–induced expression of Axin2 and Ctgf mRNA was measured by qRT-PCR in control and LRP5/6 DKO MEFs for 0, 2, 4 hr.

(C and D) Experiments similar to panels A and B were performed in 3T3-L1 cells. Data are presented as mean ± SEM.

See also Figure S3.