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. Author manuscript; available in PMC: 2015 Nov 13.
Published in final edited form as: Cell. 2015 Aug 13;162(4):727–737. doi: 10.1016/j.cell.2015.07.019

Figure 1. B cell responses to Plasmodium infection.

Figure 1

A and B- Total spleen cellularity, total B cells (A), and germinal center B cells (B) following Pc infection. Mean with standard deviation is shown; at least five mice for each time point.

C- AID expression is confined to germinal center B cells. Representative flow cytometry plots of splenocytes from Plasmodium infected AIDGFP mice. Top row shows the relative expansion of germinal center (green) over non-germinal center (red gate) B cells over time (gated on B220+). Bottom row shows the expression of AIDGFP in non-B cells (B220−, grey), non-germinal center B cells (B220+ CD38+ CD95−, red), and germinal center B cells (B220+CD38−CD95+, green). At least three mice for each time point.

D- AID protein in malaria GC B cells. Gating strategy (left) and semiquantitative Western Blot analysis (right) identifying AID in both light zone (LZ) and dark zone (DZ) cells sorted 3 weeks post inoculation. Triangles indicate threefold serial dilution. AID−/− and wild type (wt) control lanes are from in vitro activated B cells of the respective genotypes. Representative of two independent experiments.

See also Figure S1.