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. Author manuscript; available in PMC: 2016 Jan 22.
Published in final edited form as: Mol Cell. 2015 Jan 22;57(2):317–328. doi: 10.1016/j.molcel.2015.01.001

Figure 3. In-line Probing Analysis of Aptamer Mutants.

Figure 3

(A) Sequence and secondary structure of wild-type (WT) and mutant 104 Cba RNA constructs used to evaluate the importance of highly conserved nucleotides and structural elements. Mutant constructs (M1–M3) carry nucleotide changes as indicated. Other annotations are as described in the legend to Figure 2A.

(B) PAGE analysis of in-line probing reactions with WT 104 Cba RNA or mutants M1, M2, and M3. Each 5´ 32P-labeled RNA construct was incubated in the absence or presence of 10 µM ZMP. Other annotations are as described in the legend to Figure 2B.