Fig. 3. Left: EMSA DNA binding studies results for the conjugate GAGAH. (a) Lanes 1–4: [GAGAH] = 0, 500, 700, 1000 nM, and 75 nM AT·GAGA dsDNA. (b) Lanes 1–4: [GAGAH] = 0, 500, 700, 1000 nM, and 75 nM of AT·CG dsDNA. (c) Lanes 1–4: [GAGAH] = 0, 500, 700, 1000 nM, and 75 nM of GC·GAGA dsDNA. Oligonucleotide sequences (only one strand shown): AT·GAGA: 5′-CGCGTCATAATTGAGAGCGC-3′; AT·CG: 5′-CGCGTCATAATTCGCGACGC-3′; GC·GAGA: 5′-CGCGTCAT CAGCGAGAGCGC-3′. Right: fluorescence anisotropy titration of a 25 nM solution of TMR–AT·GAGA in the presence of increasing concentrations of GAGAH. The fit to a 1 : 1 binding model is also shown.
