Skip to main content
. 2015 Apr 29;43(14):6814–6826. doi: 10.1093/nar/gkv392

Figure 3.

Figure 3.

Tet1 regulates proliferation in primary cells. (A) Western blot analysis of Tet1 in control or Tet1 silenced MEF, after 24 h from silencing. Actb was used as loading control. (B) Dot-blot analysis of 5hmC and 5mC in Tet1 silenced MEFs. ssDNA was used as loading control. (C and D) FACS cell cycle and EdU incorporation analysis (at p2) in Tet1 silenced MEFs. (E) Cell growth assay in Tet1 silenced MEFs. (F) Dot-blot analysis of 5hmC and 5mC in control or Tet1 expressing MEFs at several passages. ssDNA was used as loading control. (G) FACS EdU incorporation analysis (at p2) in control or Tet1 expressing MEFs. Error bars represent the standard deviation of three independent experiments. (H) Cell growth assay in control or Tet1 expressing MEFs at several passages.