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. 2015 Apr 29;43(14):6814–6826. doi: 10.1093/nar/gkv392

Figure 4.

Figure 4.

Transcriptional regulation of Tet1.2 in proliferating cells. (A) RT-qPCR of Tet1 hnRNA in MEFs at the indicated passages. (B) Genomic view of the Tet1 promoters for the indicated ChIP-seq analysis in several mouse adult tissues. (C) qPCR of ChIP analysis for H3K4me3 and H3K27me3 in MEFs at the indicated passages. (D) qPCR of ChIP analysis for Ezh2 in MEFs at the indicated passages. (E) RT-qPCR of Suz12 and Tet1 mRNAs in control and Suz12 knockdown MEFs. (F) Western blot analysis of Tet1b in control or Suz12 silenced MEFs. Actb was used as loading control. (G) qPCR of ChIP analysis for H3K27me3 and Ezh2 in MEFs in the indicated knockdown. (H) Dot-blot analysis of 5hmC and 5mC in control or Suz12 silenced MEFs. ssDNA was used as loading control. Error bars represent the standard deviation of three independent experiments. P-value was calculated by using t-test. ** P-value < 0.001.