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. 2015 Apr 29;43(14):6814–6826. doi: 10.1093/nar/gkv392

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — Regulation of TET1 in human adult cells. (A) RT-qPCR of TET1 mRNA in HUVECs at the indicated passages (B) Dot-blot analysis of 5hmC and 5mC level in HUVECs at the indicated passages. ssDNA was used as a loading control. (C) Genomic view of the TET1 promoter for the indicated ChIP-seq analysis in hESCs and several human differentiated cells. (D) qPCR of ChIP analysis for H3K4me3 and H3K27me3 in HUVECs at the indicated passages. (E) qPCR of ChIP analysis for H3K4me3 and H3K27me3 in HUVECs at the indicated passages. Error bars represent the standard deviation of three independent experiments.