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. 2015 May 27;43(14):6902–6918. doi: 10.1093/nar/gkv525

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Caffeine inhibition of GC is independent of the DNA damage checkpoint. (A) Diagram of intrachromosomal gene conversion strain MT03. (B) Repair in MT03. HO was induced by galactose for 1 h, after which expression was shut off by addition of dextrose. (C) Repair in MT03 mec1Δ tel1Δ sml1Δ. Culture was treated as in (B). (D) Repair in caffeine treated MT03 mec1Δ tel1Δ sml1Δ. Culture was treated as in (B). 50 mM caffeine added 0.5 h after dextrose. (E) Quantification of repair in MT03 derivatives. Signal normalized to the 0 h signal.