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. 2015 May 27;43(14):6902–6918. doi: 10.1093/nar/gkv525

Figure 3.

Figure 3.

Caffeine treatment leads to Rad51 eviction. (A) Rfa1 ChIP 100 bp proximal to the DSB at MAT in a donorless strain. ChIP signal measured 100 bp distal to the DSB. Untreated samples are shown in gray, 50 mM caffeine was added 2 h after DSB induction (black). (B) Rad52 ChIP 100 bp proximal to the DSB at MAT in a donorless strain. ChIP signal measured 100 bp distal to the DSB. Untreated samples are shown in gray, 50 mM caffeine was added 2 h after DSB induction (black). (C) Rad51 ChIP 100 bp proximal to the DSB at MAT in a donorless strain. Increasing concentrations (10–50 mM in 10 mM increments) were added 2 h after DSB induction. Error bars represent ranges.