Skip to main content
. 2015 May 27;43(14):6902–6918. doi: 10.1093/nar/gkv525

Figure 4.

Figure 4.

Caffeine treatment leads to Rad51 eviction independently of factors known to displace Rad51. (A) Rad51 ChIP 5 kb proximal to the DSB at MAT in a donorless strain. Increasing concentrations (10–50 mM in 10 mM increments) were added 2 h after DSB induction. Untreated n = 13, 10–40 mM caffeine n = 3, 50 mM caffeine n = 5. (B) Rad51 ChIP 5 kb proximal to the DSB at MAT in a mec1Δ tel1Δ sml1Δ donorless strain. (C) Rad51 loading in an srs2Δ strain. 50 mM caffeine added 2 h after HO induction. ChIP signals measured 5 kb from the DSB. Error bars represent ranges. (D) Rad51 loading in a rad54Δ rdh54Δ uls1Δ strain. 50 mM caffeine added 2 h after HO induction. ChIP signals measured 5 kb from the DSB. Error bars represent ranges. (E) Rad51 loading in an atg1Δ strain. 50 mM caffeine added 2 h after HO induction. ChIP signals measured 5 kb from the DSB.