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. 2015 May 27;43(14):6902–6918. doi: 10.1093/nar/gkv525

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 9. — Rad51 overexpression rescues the Rad51 filament and GC. (A) Rad51 ChIP 5 kb from a DSB at MAT in a donorless strain transformed with a 2μ plasmid containing RAD51 under the ADH1 constitutive promoter (pJH657). Error bars represent ranges. (B) Repair in tGI354 with RAD51 overexpression vector. Culture split 1 h after HO induction, half treated with 50 mM caffeine. (C) Quantification of repair in tGI354 untreated (diamonds) tGI354 treated with 50 mM caffeine 1 h after HO induction (black squares), tGI354 with RAD51 overexpression vector (circles) and tGI354 with RAD51 overexpression vector treated with 50 mM caffeine 1 h after HO induction (triangles). Signal normalized to the maximum signal of the untreated cultures and 0 h. Error bars represent standard deviations.