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. 2015 Jun 27;43(14):6677–6691. doi: 10.1093/nar/gkv641

Figure 5.

Figure 5.

The effects of SYSU-ID-01 on 14 NM23-H2-associated genes. The mRNA levels of 14 genes in untreated HeLa cells (left), 10 μM SYSU-ID-01-treated HeLa cells (right), and NM23-H2 knockdown HeLa cells (middle). The detected genes included 14 NM23-H2-associated genes, which showed significant changes in transcription levels during the knockdown of the NM23-H2 genes. Total RNA was purified and then reverse transcribed and analysed by RT-PCR for the indicated genes. Expression data for each gene were derived by quantitatively calculating the optical density of the left electrophoresis gels and graphing the results (original full gels were shown in Supplementary Figure S9). The y-axis indicates the relative expression data for each gene normalized to the untreated samples (n = 3; error bars, S.E.M.). Data from untreated samples and samples treated for 72 h with 10 μM SYSU-ID-01 or NM23-H2 siRNA were shown for each gene.