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. 2015 Jul 2;43(14):7152–7161. doi: 10.1093/nar/gkv655

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Dual transcription and splicing regulation eliminates leaky hypersensitive response. (A) Nicotiana benthamiana leaf with three spots transiently co-transformed with Agrobacterium tumefaciens to introduce SuperPromoter::Bs2-HA resistance gene and either (i) constitutive (35S) avrBs2-HA, (ii) Dex-inducible (6xUAS) avrBs2-HA or (iii) Dex-inducible avrBs2-HyP5SM-HA. The leaf was not treated with Dex and OsL5 is not co-expressed. (B) Scheme representing how a two input system that combines promoter and suicide exon elements to control transcription and alternative splicing, respectively, results in tighter regulation of phenotype compared to a one input system (promoter alone). See Supplementary Figure S1 for a related scheme showing the case in the presence of inputs.