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. 2015 Jul 2;43(14):7152–7161. doi: 10.1093/nar/gkv655

Figure 3.

Figure 3.

The two-input regulatory system overcomes both leaky transcription and exon skipping to tightly control protein expression and HR phenotype. (A) Anti-HA western blot to detect protein expression from avrBs2-HA or avrBs2-HyP5SM-HA constructs induced or mock induced at 18 hpi, with tissue collected at 26 hpi. Shown at the bottom is Ponceau S staining of the nitrocellulose membrane to visualize the large subunit of RuBisCo as a loading control. (B) Induction of the HR phenotype from the effector construct avrBs2-HyP5SM-HA requires co-transformation with Bs2-HA and either co-transformation with OsL5, treatment with 30 μM Dex or both. Neither chlorosis nor HR is visible in the absence of both inputs. Lanes 4 and 9 are empty.