Table 2. Gene ontology (GO) categories enriched during the procyclic to metacyclic transition.
| Metacyclic promastigotes, downregulated | ||
| GO ID | GO term | P-value |
| GO:0015986 | ATP synthesis coupled proton transport | 7.36e-11 |
| GO:0005737 | cytoplasm | 5.33e-09 |
| GO:0015991 | ATP hydrolysis coupled proton transport | 2.00e-08 |
| GO:0046961 | proton-transporting ATPase activity, rotational mechanism | 2.79e-08 |
| GO:0004812 | aminoacyl-tRNA ligase activity | 6.96e-08 |
| GO:0006418 | tRNA aminoacylation for protein translation | 6.96e-08 |
| GO:0046933 | proton-transporting ATP synthase activity, rotational mechanism | 7.26e-08 |
| GO:0044267 | cellular protein metabolic process | 1.41e-07 |
| GO:0003746 | translation elongation factor activity | 1.55e-07 |
| GO:0005634 | nucleus | 3.38e-07 |
| GO:0006260 | DNA replication | 1.25e-06 |
| GO:0005525 | GTP binding | 1.42e-06 |
| GO:0003677 | DNA binding | 1.94e-06 |
| GO:0003924 | GTPase activity | 2.64e-06 |
| GO:0043234 | protein complex | 1.64e-05 |
| GO:0051258 | protein polymerization | 1.64e-05 |
| GO:0003743 | translation initiation factor activity | 1.74e-05 |
| GO:0045261 | proton-transporting ATP synthase complex, catalytic core F(1) | 5.23e-05 |
| GO:0006457 | protein folding | 7.78e-05 |
| GO:0051082 | unfolded protein binding | 8.59e-05 |
| GO:0005874 | microtubule | 1.07e-04 |
| GO:0004298 | threonine-type endopeptidase activity | 1.37e-04 |
| GO:0005839 | proteasome core complex | 1.37e-04 |
| GO:0051603 | proteolysis involved in cellular protein catabolic process | 1.37e-04 |
| GO:0006334 | nucleosome assembly | 1.47e-04 |
| GO:0006413 | translational initiation | 1.93e-04 |
| GO:0046982 | protein heterodimerization activity | 2.60e-04 |
| GO:0003887 | DNA-directed DNA polymerase activity | 3.45e-04 |
| GO:0006414 | translational elongation | 6.65e-04 |
| GO:0005198 | structural molecule activity | 9.43e-04 |
| GO:0004175 | endopeptidase activity | 9.86e-04 |
| GO:0016272 | prefoldin complex | 1.07e-03 |
| GO:0050660 | flavin adenine dinucleotide binding | 1.09e-03 |
| Metacyclic promastigotes, upregulated | ||
| GO ID | GO term | P-value |
| GO:0004674 | protein serine threonine kinase activity | 4.61e-22 |
| GO:0006468 | protein phosphorylation | 1.91e-21 |
| GO:0004672 | protein kinase activity | 8.67e-20 |
| GO:0004713 | protein tyrosine kinase activity | 1.28e-18 |
| GO:0005524 | ATP binding | 3.82e-11 |
| GO:0006950 | response to stress | 2.77e-10 |
| GO:0016791 | phosphatase activity | 1.63e-04 |
GOseq (58) was used to perform gene ontology analysis using differentially expressed genes identified as the parasite undergoes metacyclogenesis. Using a P-value cut off of <0.05, a total of 33 GO categories were enriched among genes that were downregulated in metacyclic promastigotes and a total of seven GO categories were enriched among genes that were upregulated in metacyclic promastigotes. The differentially expressed genes corresponding to each enriched GO category are reported in Dataset S4.