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. 2015 Jul 2;43(14):7005–7020. doi: 10.1093/nar/gkv676

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Star-PAP S6 phosphorylation together with PIPKIα determines specificity of target mRNA expression. qRT-PCR analysis of (A) Star-PAP target mRNAs as indicated with total RNA isolated from HEK 293 cells after Star-PAP knockdown rescued with stable expression of WT or S6A mutant Star-PAP; (B) after knockdown of PIPKIα or Star-PAP; (C) qRT-PCR as in (A) with select Star-PAP target mRNAs as indicated and (D) more Star-PAP target mRNAs as in (A) to screen for S6-phospho dependent target mRNAs. (E) Western blot analysis showing knockdowns of Star-PAP and PIPKIα. Wherever siRNA (−) is indicated in the figure, we have used control scrambled siRNA.