Figure 1. p75^NTR regulates miR-503 expression.

(a) HUVECs were exposed to high glucose (HG), cultured in normal glucose (NG) or osmotic control (Cont; L-Glucose) conditions for 24 h, and p75^NTR expression was analysed using qPCR. **P<0.01 versus NG or Cont (n=3). (b) Representative western blot images and protein quantification of p75^NTR and Tubulin expression in total cell extracts. **P<0.01 versus NG or Cont (n=3). (c) Microarray data validation using qPCR. HUVECs were transduced with Ad.Null or Ad.p75 and qPCR was carried out to measure the expression of top-ranked miRNAs. (d) Expression of precursor and mature miR-503 for c,d; *P<0.05, **P<0.01 versus Ad.Null (n=3). (e) Relative expression of miR-503 in HUVECs transfected with scrambled oligos (scr oligos) or short interfering RNA (siRNA)-p75 oligos and then cultured in Cont or HG for 24 h. **P<0.01 (n=5). Unpaired two-tailed Student's t-test or Mann–Whitney nonparametric test was applied. Differences among groups were analysed using two-way analysis of variance followed by Bonferroni post hoc test. All values are mean±s.e.m. of three independent experiments.