FIGURE 3.

Shared and unique structures plots showing comparison of changes to intracellular metabolites caused by Al (A,B) and Cu (C,D) in cultures of P. pseudoalcaligenes KF707 grown on either Sc or Bp as the sole carbon source. Coordinates were determined by the VIP of each metabolite, as obtained from OPLS-DA models comparing control and metal exposed samples for either Sc (y-axes) or Bp (x-axes). Metabolites with a VIP ≥ 0.8 (dashed lines) indicate a significant change occurred in the metal exposed samples, those that were below in both cases were omitted. The association of each metabolite with control or metal exposed samples was determined using p(corr), which indicates the degree of correlation of the metabolite with a sample type. Shapes were assigned that indicate how the metabolite was altered by metal exposure: increased in both Bp and Sc (squares), increased in Bp but decreased in Sc (diamonds), increased in Sc but decreased in Bp (triangle up) and decreased in both (triangle down).