Fig. 6.

Gene expression of hPXR transgenic mouse hepatic enzymes involved in EtOH metabolism. Male and female hPXR mice were orally administered saline (control; □) or EtOH (4.5 g/kg; ▪) every 12 hours for a total of three doses and were killed 4 hours after the final dose. Total hepatic RNA was isolated and cDNA prepared using Tetro cDNA Synthesis Kit (Bioline). (A) Adh1, (B) Adh4, (C) Adh5, and (D) Aldh2, were determined using proprietary Taqman Gene Expression Assays purchased from Applied Biosystems/Life Technologies as described in Materials and Methods. Data represent the mean ± S.E.M. (n = 5–6). ^#P < 0.05 between male and female mice treated with saline (control) and EtOH.