Fig. 7.

Immunoblot analysis of hepatic EtOH-metabolizing enzymes in hPXR mice. Male and female hPXR mice were orally administered saline (control; □) or EtOH (4.5 g/kg; ▪) every 12 hours for a total of three doses and were killed 4 hours after the final dose. Western blots of liver homogenate (40 μg/lane) were probed with antibodies to ADH1 (A), ALDH2 (B), catalase (C), CYP2E1 (D), or CYP3A11 (E). Bands were quantified and normalized to α-tubulin. Data represent the mean ± S.E.M. of two independent experiments of 3–4 mice/group. *P < 0.05 between male and female mice treated with saline (control); ^#P < 0.05 between male and female mice treated with saline (control) and EtOH; ^†P < 0.05 between male and female mice fed EtOH.