TABLE 1.
Sequences of primers used for real-time quantitative PCR
| Name | Sequence | Accession No. |
|---|---|---|
| PPARα | ||
| Sense | GATTCAGAAGAAGAACCGGAACA | NM011144 |
| Antisense | TGCTTTTTCAGATCTTGGCATTC | |
| PPARγ | ||
| Sense | CCCAATGGTTGCTGATTACAAA | NM011146 |
| Antisense | GAGGGAGTTAGAAGGTTCTTCATGA | |
| SREBP-1c | ||
| Sense | CATGCCATGGGCAAGTACAC | NM011480 |
| Antisense | TGTTGCCATGGAGATAGCATCT | |
| CPT1 | ||
| Sense | CGATCATCATGACTATGCGCTACT | NM013495 |
| Antisense | GCCGTGCTCTGCAAACATC | |
| ACOX-1 | ||
| Sense | TTTGTTGTCCCTATCCGTGAGA | NM 015729 |
| Antisense | GCCGATATCCCCAACAGTGA | |
| L-FABP | ||
| Sense | TGCATGAAGGGAAGAAAATCAAA | NM017399 |
| Antisense | CCCCCAGGGTGAACTCATT | |
| MTTP | ||
| Sense | CCGCTGTGCTTGCAGAAGA | NM008642 |
| Antisense | TTTGACACTATTTTTCCTGCTATGGT | |
| CD36 | ||
| Sense | TCCAGCCAATGCCTTTGC | NM007643 |
| Antisense | TGGAGATTACTTTTTCAGTGCAGAA | |
| ACC-1α | ||
| Sense | ATGTCCGCACTGACTGTAACCA | NM133360 |
| Antisense | TGCTCCGCACAGATTCTTCA | |
| FAS | ||
| Sense | CCCGGAGTCGCTTGAGTATATT | NM007988 |
| Antisense | GGACCGAGTAATGCCATTCAG | |
| SCD1 | ||
| Sense | CGTTCCAGA ATGACGTGTACGA | NM009127 |
| Antisense | AGGGTCGGCGTGTGTTTC | |
| PEMT | ||
| Sense | TCTGCATCCTGCTTTTGAACA | NM008819 |
| Antisense | TGGGCTGGCTCATCATAGC | |
| GAPDH | ||
| Sense | TGTGTCCGTCGTGGATCTGA | NM001001303 |
| Antisense | CCTGCTTCACCACCTTCTTGA |
ACC-1α, acetyl-CoA carboxylase 1α; ACOX-1, acyl-CoA oxidase 1; CPT1, carnitine palmitoyltransferase 1; FAS, fatty acid synthase; L-FABP, liver-type fatty acid-binding protein; SCD1, stearoyl-CoA desaturase-1.