Table 2.
Loci initially explored in the study and location on barley chromosomes
Locus name | RAP2 description | Chromosomea | No. of reads | Coverage mean ± 1 SD |
---|---|---|---|---|
TNAC1035 | Kinesin, motor region domain containing protein | 1H | 62,836 | 518 ± 340.0 |
TNAC1142 | Similar to COP9 signalosome complex subunit 5b | 2H (?) | 51,409 | 305 ± 214.4 |
XYL | Xylose isomerase | 2HS | 87,926 | 547 ± 329.8 |
TNAC1364 | Ubiquitin domain containing protein | 3HL | 53,286 | 334 ± 243.1 |
NUC | Nucellin | 4HL | 31,916 | 260 ± 275.4 |
TNAC1403 | Similar to SAC domain protein 1 (FIG4-like protein AtFIG4) | 4HL | 151,385 | 490 ± 334.9 |
TNAC1463 | Proteasome subunit beta type 2 | 4HS | 97,610 | 380 ± 389.5 |
BLZ1 | Barley leucin zipper | 5HL | 56,064 | 376 ± 236.8 |
TNAC1610 | Peptidase S16, ATP-dependent protease La family protein | 5HL | 71,133 | 374 ± 342.0 |
TOPO6 | Topoisomerase VI subunit B | 5HL | 27,416 | 226 ± 159.1 |
TNAC1577b | Conserved hypothetical protein | 5HL (?) | Na | Na |
TNAC1497 | Similar to Nucleoside diphosphate kinase II, chloroplast precursor | 5HS | 71,439 | 502 ± 327.2 |
TNAC1740 | Heat shock protein Hsp70 family protein | 6HL | 49,843 | 297 ± 241.7 |
TNAC1781b | Beta 5 subunit of 20S proteasome | 7HS (?) | Na | Na |
matK | Maturase K | cp-LSC | 179,382 | 426 ± 284.2 |
ndhFb | Subunit 6 of NADH-dehydrogenase | cp-SSC | Na | Na |
aChromosome locations were checked by blasting sequences obtained against the barley genome, no significant result was obtained from TNAC1142, locations for this locus and the other loci missing were inferred from synteny with rice and wheat. Questionmarks refer to not safely determined positions.
bndhF was excluded because of large amount of missing data and TNAC1577 and TNAC1781 were not single-copy loci and difficult to sequence (large homopolymer regions).