Table 1.
IL-6 ELISA data obtained from dendrimer plate, kit plate, by physical adsorption and dendrimer plate with luminol detection.
| concentration [pg ml-1] | Dendrimer Plate TMB detection [O.D.] | IL-6 kit TMB detection [O.D.] | Physical Adsorption TMB detection [O.D.] | Dendrimer Plate Luminol detection [R.L.U.] |
|---|---|---|---|---|
| 300 | 1.975 | 1.647 | 1.813 | – |
| 100 | 0.697 | 0.643 | 0.931 | 7420.6 |
| 25 | 0.213 | 0.151 | 0.395 | 3002.0 |
| 6.25 | 0.085 | 0.059 | 0.336 | 1831.8 |
| 1.56 | 0.05 | 0.035 | 0.323 | 1435.8 |
| 0.78 | 0.042 | 0.032 | 0.321 | 1331.5 |
| 0.39 | 0.038 | – | 0.322 | 1247.9 |
| 0.195 | 0.035 | – | 0.321 | 1226.1 |
| 0.098 | – | – | – | 1205.6 |
| 0 | 0.034 | 0.031 | 0.319 | 1109.6 |
|
| ||||
| STD, [0]IL-6 | 0.00346 | 0.0025 | 0.009 | 33.4 |
|
| ||||
| RSD [%], [0]IL-6 | 10.1 | 8.1 | 7.8 | 6.6 |
|
| ||||
| R² | 0.999 | 0.997 | 0.992 | 0.995 |
|
| ||||
| LLD[a] | 0.04438 | 0.0385 | 0.346 | 1209.7 |
|
| ||||
| Sensitivity [pg ml-1] | < 0.29 | < 0.87 | < 5.75 | < 0.13 |
[a]
The lower limit of detection (LLD) of IL-6 was determined by adding three STD to the mean OD of five zero standard replicates and calculate the corresponding concentration from the standard curve.