Table 2.
Dilution | Dendrimer Plate | Kit Plate | |||
---|---|---|---|---|---|
Expected [pg ml-1] | Observed [pg ml-1] | [%] | Observed [pg ml-1] | [%] | |
neat | 200 | 193.9 | 97 | 193.5 | 96.8 |
1:2 | 100 | 96.1 | 96.1 | 109.4 | 109.4 |
1:4 | 50 | 59.2 | 118.4 | 53.1 | 106.2 |
1:8 | 25 | 27.2 | 108.8 | 25.4 | 101.6 |
1:16 | 12.5 | 12.1 | 96.8 | 12.1 | 96.8 |
Human serum samples (from a non-pregnant woman) were spiked with high concentrations of IL-6 and diluted with assay diluent to produce samples with values within the dynamic range of the assay.