Fig. 4.

Regulation of cellular infiltration (CD4 and CD8 Tcells, and macrophages) in heart allogarfts by anti-OX40L mAb treatment. Cardiac allografts in Rag-1^−/− B6 recipients were harvested on POD 100. The CD4, CD8 and Mac-1 expressing cells in the sections of the grafts were localized by immunohistochemistry. Data are presented as a representative immunoperoxidase staining for each of CD4⁺, CD8⁺, and Mac-1⁺ cells. a, c and e A typical microscopic view of CD4⁺ T cells, CD8⁺ T cells, or Mac-1⁺/macrophages in the sections of the grafts from Rag-1^−/− recipients harboring T_mem cells (Group 2, untreated, n =8). The red arrows indicated positively stained (brown) cells. b, d and f A typical microscopic view of CD4, CD8, or Mac-1 staining in the sections of the grafts from Rag-1^−/− recipients harboring T_mem cells and receiving anti-OX40L mAb treatment (Group 3, n =8). Cytoplasm: blue. Nucleus: dark blue