Figure 3.

(A) PLGA-OP treatment of RAGxCγ double mutant mice bearing heterotopic xenografts of pancreatic PANC1 tumors. Cells at 0.5×10⁶ in 0.2 mL were implanted subcutaneously in the right back flank of these mice. Twice a week following implantation of the PANC1 cancer cells, each mouse was monitored for tumor volume ((width squared/2) × length) at the site of implantation. Mice were surgically implanted with sterilized PLGA-empty (four mice) and PLGA-OP (four mice cylinders) containing 20 mg OP at day 35 post-implantation of cancer cells when the tumor volume reached approximately 50–120 mm³. (B) Graph of mean ± SEM of live necropsy tumor weight per mouse body weight. Statistical analysis using unpaired t-test was carried out using GraphPad Prism, and the results were compared with untreated control cohort.

Abbreviations: OP, oseltamivir phosphate; PLGA, poly (lactic-co-glycolic acid); SEM, standard error of the mean; RAG, recombinase activating gene.