Figure 6.

Necropsy liver (A) and lung (B). RAGxCγ double mutant mice were implanted with 0.5×10⁶ PANC1 pancreatic cancer cells subcutaneously on the rear flank and PLGA-empty and PLGA-OP 20 mg cylinders were surgically implanted at day 35 post-implantation when tumors reached 100–120 mm³. Paraffin-embedded tissue sections (5 μm) on glass slides were processed for HE staining for each mouse necropsied at end point of the study. Stained tissue sections were photographed using a Zeiss Imager M2 fluorescence microscope at 400× magnification. Images are representative of at least five fields of view from two tissue sections. Metastatic tissue clusters were microscopically counted per tissue sections (5 μm) and plotted in the graph.

Abbreviations: OP, oseltamivir phosphate; PLGA, poly (lactic-co-glycolic acid); RAG, recombinase activating gene.