Table 1.
All heart functional recovery parameters
| Time (min) | Ischemic conservation | Groups | LVSP (mmHg) | LVEDP (mmHg) | LVDP (mmHg) | HR (beats·min-1) |
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| KH+O2 | Rep-40 | 99±4 | 3±1 | 96±4 | 167±5 | |
| Air | Rep-40 | 115±7 | 1±1 | 115±6 | 162±10 | |
| Basal | KH+O2 | Rep-60 | 97±5 | 2±1 | 95±5 | 172±21 |
| KH+N2 | Rep-60 | 111±10 | 2±2 | 109±9 | 162±9 | |
| Perfusion | Air | Rep-60 | 112±7 | 2±1 | 111±7 | 158±7 |
| KH+O2 | Rep-90 | 101±5 | 4±1 | 97±6 | 165±43 | |
| Air | Rep-90 | 109±5 | 1±0.7 | 108±6 | 169±9 | |
|
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| Reperfusion | ||||||
|
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| KH+O2 | Rep-40 | 41±10* | 16±10 | 25±2* | 168±17 | |
| Air | Rep-40 | 112±5 | 1±1 | 112±4 | 155±6 | |
| KH+O2 | Rep-60 | 37±4 | 8±3 | 29±2 | 165±5 | |
| 20 min | Air | Rep-60 | 115±15 | 1±1 | 115±15 | 150±5 |
| KH+N2 | Rep-60 | 53±5+ | 3±2 | 50±5+ | 160±12 | |
| KH+O2 | Rep-90 | 44±16* | 20±15 | 24±3* | 153±21 | |
| Air | Rep-90 | 111±6 | 1±1 | 111±5 | 161±8 | |
| KH+O2 | Rep-40 | 43±6* | 13±7 | 30±3* | 165±5 | |
| Air | Rep-40 | 116±13 | 1±1 | 115±24 | 156±19 | |
| KH+O2 | Rep-60 | 43±3* | 7±2 | 36±2* | 168±5 | |
| 40 min | Air | Rep-60 | 114±13 | 1±1 | 113±13 | 158±10 |
| KH+N2 | Rep-60 | 68±8+ | 3±2 | 65±7+ | 174±5 | |
| KH+O2 | Rep-90 | 43±10* | 12±10 | 31±3* | 167±8 | |
| Air | Rep-90 | 108±7 | 1±0.64 | 108±7 | 160±8 | |
| KH+O2 | Rep-60 | 45±6* | 11±5 | 33±4 | 165±6 | |
| Air | Rep-60 | 94±11 | 1±0.4 | 94±11 | 180±9 | |
| 60 min | KH+N2 | Rep-60 | 74±8+ | 2±2 | 72±7+ | 162±16 |
| KH+O2 | Rep-90 | 52±11* | 19±11 | 33±7* | 170±9 | |
| Air | Rep-90 | 94±9 | 1±0.34 | 94±9 | 176±9 | |
| 90 min | KH+O2 | Rep-90 | 53±16* | 23±15 | 30±5* | 162±3 |
| Air | Rep-90 | 79±8 | 1±0.33 | 178±8 | 161±8 | |
All heart function recovery parameters were better preserved when hearts were suspended in air or immersed in nitrogen-KH solution (KH+N2) compared to hearts immersed in oxygenated-KH solution (KH+O2) during the normothermic ischemic period. Time course of cardiac function recovery parameters, including left ventricular systolic pressure (LVSP); left ventricular end-diastolic pressure (LVEDP); left ventricular developed pressure (LVDP) and heart rate (HR) of the three groups: 1) hearts immersed in KH+O2, 2) hearts immersed in KH+N2, and 3) in hearts suspended in air. Note that hearts immersed in KH+O2 and hearts suspended in air where subjected to three different reperfusion durations (Rep-40, Rep-60, and Rep-90), while hearts immersed in KH+N2 received only 60 minutes of reperfusion. Values are mean ± SEM.;
P<0.05 hearts immersed in KH+O2 versus hearts maintained in air;
P<0.05 hearts immersed in KH+O2 versus hearts immersed in KH+N2 (n = 4-10/group).