Figure 1.

Auto-Ab identification methods used in this study. (A) Sera from the 34 rheumatoid arthritis (RA) patients with or without ILD were analyzed for anti-HLA Ab profiles. (b) Sera from the 15 collagen disease patients with acute-onset diffuse ILD (AoDILD) were collected on admission and in the stable state. Two samples were collected from each patient. These individual sera were analyzed for anti-HLA Ab profiles or granulocyte immunofluorescence test (GIFT). (C) The sera from these patients either with AoDILD or in the stable state were combined; the two pooled sera at these two states were screened for the auto-Ab profiling in ProtoArray and AlphaScreen systems. (D) These individual sera were analyzed by glutathione S-transferase (GST) capture enzyme-linked immunosorbent assay (ELISA). Analyses of individual sera are written in black and those of pooled sera in red.