Table 2.
Linearity, limits of detection (LOD) and quantification (LOQ) results for alcohol markers in meconium
| Analyte | LOD ng/g (nmol/g) | LOQ ng/g (nmol/g) | Linear range ng/g (nmol/g) | y-intercept (mean ± SD, n=5) | Slope (mean ± SD, n=5) | R2 (range, n=5) |
|---|---|---|---|---|---|---|
| Ethyl laurate | 50 (0.219) | 50 (0.219) | 50–5,000 (0.219–21.9) | −0.003±0.017 | 0.273±0.133 | 0.990–0.997 |
| Ethyl myristate | 25 (0.098) | 25 (0.098) | 25–5,000 (0.098–19.5) | 0.009±0.014 | 0.864±0.155 | 0.996–0.999 |
| Ethyl linolenate | 15 (0.049) | 25 (0.082) | 25–5,000 (0.082–16.3) | −0.007±0.012 | 1.081±0.236 | 0.997–1.000 |
| Ethyl palmitoleate | 15 (0.053) | 25 (0.088) | 25–5,000 (0.088–17.7) | −0.181±0.149 | 3.466±1.169 | 0.990–0.996 |
| Ethyl arachidonate | 15 (0.045) | 25 (0.075) | 25–2,500 (0.075–7.5) | 0.095±0.038 | 4.000±0.088 | 0.997–1.000 |
| Ethyl linoleate | 15 (0.049) | 25 (0.081) | 25–5,000 (0.081–16.2) | 0.040±0.017 | 0.905±0.029 | 0.997–0.999 |
| Ethyl palmitate | 50 (0.176) | 50 (0.176) | 50–5,000 (0.176–17.6) | 0.125±0.029 | 1.342±0.036 | 0.993–0.997 |
| Ethyl oleate | 15 (0.048) | 25 (0.081) | 25–5,000 (0.081–16.1) | 0.199±0.088 | 0.940±0.019 | 0.995–0.999 |
| Ethyl stearate | 50 (0.160) | 50 (0.160) | 50–5,000 (0.160–16.0) | 0.295±0.462 | 1.204±0.071 | 0.997–0.999 |
| Ethyl sulfate (EtS) | 2.5 (0.020) | 2.5 (0.020) | 2.5–500 (0.020–3.96) | 0.308±0.047 | 1.024±0.066 | 0.992–0.996 |
| Ethyl glucuronide (EtG) | 5 (0.023) | 5 (0.023) | 5–1,000 (0.023–4.51) | 0.446±0.096 | 1.240±0.250 | 0.995–1.000 |