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. Author manuscript; available in PMC: 2015 Aug 17.
Published in final edited form as: Mol Ther. 2008 Jan 29;16(3):580–589. doi: 10.1038/sj.mt.6300404

Figure 5. Cytokine profiling of the engineered T cells.

Figure 5

Cytokine release assays were performed by coculture of 5 × 104 T cells with 2 × 104 target cells per well in duplicate in 96-well flat-bottom plates at a final volume of 200 μl T-cell media. After 24 hours, the supernatant was assayed for cytokine production using Fluorokine MAP Immunoarray. Similar data were also obtained from another assay. CM-CSF, granulocyte-macrophage colony-stimulating factor; IFN-γ, interferon-γ; IL-2, interleukin-2; TNF-α, tumor necrosis factor-α; UCB, umbilical cord blood.