Table 1.
Mapping of transposition sites in SB-engineered T cells
| Clones | IR/DR (L) | Chromosome location | Clones | IR/DR (R) | Chromosome location |
|---|---|---|---|---|---|
| PBL2-19BBζ/CD20 | |||||
| 1 | GATGGACAGATAGATGAAC AGATGAACACATACAGTTG | 6 p21.32 | 10 | CAACTGTAACTTACCTTTC TGCTTCAGAAACCCCGCCC | 1 q42.12 |
| 2 | AGGTACAAAAACCTATCAA GAATTCCATATTACAGTTG | 5q31.1 | 11 | CAACTGTACATCCATGGT CCCTTAAGCGGAGCCCTA | ND |
| 3 | TATCTGTATATGAGTGTGC GTATAGGTACATACAGTTG | 9 q22.2 | 12 | CAACTGTATATGCTTAAAGC AAAAGGAACAAAGAGAGG | 2 q37.3 |
| 4 | CACAAAAGAAACATAAGCC AGAAGTACAATTACAGTTG | 14q24.3 | 13 | CAACTGTAAAAGCTATTTC TATAACTGCTCTCTGCCAA | 1 p21.1 |
| 5 | GAATGTAAGTGAGGTCCAG GTGAGCACATTTACAGTTG | llq23.3 | 14 | CAACTGTATCTATCTATGT ATCCATGGTCCCTTAAGCG | Ypll.32orXp22.33 |
| 6 | ATTTGTTACATATAGCACCC CTGGGAAATGTACAGTTG | 8 q24.13 | 15 | CAACTGTATTTCTGATGCC CTTCTCCTTGTCTGCCCCA | 2 q37.3 |
| 7 | TAAAAAGGAAGGAGGAAAA TCATACACCAATACAGTTG | 18qll.l | 16 | CAACTGTATATGTGAATAG TGTAGATTGTATGGGTTCT | 8 q24.22 |
| 8 | CAATCCGTAACTGTGGGTA GGTAGAGTTGTTACAGTTG | 14 q24.1 | 17 | CAACTGTAAAAGCTATTTC TATAACTGCTCTCTGCCAA | 1 p21.1 |
| 9 | CAGGCCACACCCTGGCAGT CACAAAGCACATACAGTTG | 9 q33.3 | 18 | CAACTGTACATGGTCC CTTAAGCGGAGCCCT | 14 q22.2 |
| UCBCD3+-19BBζ/CD20 | |||||
| 21 | ACCTCTGTTGCTGGAGAAA ATTAAACCACATACAGTTG | 2q31.3 | 28 | CAACTGTATATAGTCAGGG TCTTGCTCTGTTGCTCACA | Xq22.3 |
| 22 | TAGTTCAAAATAGGGAGAG CAAATTTCAAATACAGTTG | 1 q21.3 | 29 | CAACTGTAAATCTTCCCAT GGTCCCTTAAGCGGAGCCC | ND |
| 23 | ATGGTCCTACTGGGAGGGC CAGCAGTCTTATACAGTTG | 2 q22.3 | 30 | CAACTGTATATTTCCCTGA AAAGAAGTAATTCCCATTA | 6q26 |
| 24 | CTTTGGCCTGTGGAAAATC TCTATCACATATACAGTTG | 19 ql3.41 | 31 | CAACTGTATATACACATGG TCCCTTAAGCGGAGCCCT | ND |
| 25 | TGGGAGGGGCTCACAGTGG CTTCAAAGATATACAGTTG | 5 q35.3 | 32 | CAACTGTATTGCCACTTAC TTTTGCCAACAGGGTAATA | 2pll.2 |
| 26 | GTCACTTTCCAGGAGAAAG GGTGGCCCATATACAGTTG | 3 q25.1 | 33 | CAACTGTAAGTGTCCTTCA GTGGATGAACACATAAAGA | 18pll.21 |
| 27 | AGAGATAGGCTTTCTAACT GATTAAACACATACAGTTG | 2 q32.3 | 34 | CAACTGTACCAAATTCTAG TAAAACCGTTTGTTTTTCA | 7qll.23 |
Bfal or N/alll was used for digesting genomic DNA from engineered PBL and umbilical cord blood T cells to recover left and right inverted/directed repeat (IR/DR): chromosome junction sequences, respectively. Sequences in bold print represent the transposon junction region. ND, not determined because of short genomic sequences from cloned polymerase chain reaction products. The length of genomic sequences was between 25 and 263 base pairs (bp).