. Author manuscript; available in PMC: 2015 Aug 17.

Published in final edited form as: Nat Rev Nephrol. 2015 Apr 14;11(8):451–464. doi: 10.1038/nrneph.2015.39

Table 1.

Articles using plasma membrane anchored chimeric proteins to study the function of the COOH-terminal tail of Polycystin 1

Reference	Model system	Findings
Sutters 2001¹⁷	M1 cells; dexamethasone inducible sIg7-PC1-193	sIg7-PC1-193 transforms the response to cAMP from growth inhibition to growth stimulation.
Vandorpe 2002¹⁸	Xenopus oocytes and HEK cells; CD16.7-PC1(CTT constructs)	CD16.7-PKD1(1–226), (115–226), (115–203) or (115–189), but not CD16.7-PKD1(1–92), (1–115), (1–155) or CD16.7 (control) up-regulate a nonselective Ca2+-permeable cation conductance.
Aguiari 2003¹⁹	HEK-293 and HeLa cells; Trk-PC1(CTT constructs)	Trk-PC1-1-226 or 26-226 enhance ATP-evoked (HEK) and histamine-evoked (HeLA) cytosolic Ca2+ transients (upregulation of PC2 or other intracellular cation channel activity?).
Hooper 2003²¹	M1 cells; dexamethasone inducible sIg7-PC1-193	sIg7-PC1-193 enhances ATP induced, but not forskolin induced, chloride secretory currents.
Wildman 2003²⁰	M1 cells; dexamethasone inducible sIg7-PC1-193	sIg7-PC1-193 prolongs the decay phase of ATP induced Ca2+ transients and the duration of ATP stimulated Cl-conductance.
Puri 2004²²	HEK cells; sIg7-PC1(CTT constructs)	PC1 constructs containing the heterotrimeric G protein binding and activation domain activate Gαq and PLC, releasing of Ca2+ from intracellular stores, activating store-operated Ca2+ entry, and activating calcineurin and NFAT.
Manzati 2005²³	HEK-293 cells; Trk-PC1(26–226)	Trk-PC1(26–226) expressing cells have higher basal Ca2+ concentration and 1% FBS or ATP induced Ca2+ transients and proliferative responses.
Xu 2007²⁴	NK cells; CD16.7-PC1(115–226).	CD16.7-PKD1(115–226) accelerates the decay of flow-induced Ca2+ transients.