Table 2. Multiple myeloma cell and normal plasma cell counts in bone marrow and peripheral blood samples one day before and 7 days after high dose melphalan and autologous stem cell transplantation.
| One day before HDM | 7 days after HDM and ASCT | 3 months after HDM and ASCT | |
|---|---|---|---|
| Bone marrow | |||
| Patients with positive MRD the day before HDM | |||
| Number of patients evaluated | 18 | 18/18 | 12/18 |
| Bone marrow cell count (106 cells/mL) | 8.4 (3.7-27.0) | 0.3 (0.1-3.6) | 9.8 (3.9-20.6) |
| Detection of MMCs | Detected in 18/18 samples | Detected in 17/18 samples | Detected in 9/12 samples |
| MMC count (cells/μL) | 71.7 (0.4-285.5) | 5.5 (0-76.9) | 2.4 (0-68.3) |
| N-PC count (cells/μL) | 4.3 (0.7-17.0) | 3.3 (0.3-32.2) | 6.1 (0.3-21.5) |
| B-lymphocyte count (cells/μL) | 22.7 (4.8-126.6) | 0.9 (0-14.2) | 184.4 (58.5-368.7) |
| Patients with negative MRD the day before HDM | |||
| Number of patients evaluated | 9 | 9/9 | 7/9 |
| Bone marrow cell count (106 cells/mL) | 9.4 (6.0-28.9) | 0.9 (0.2-2.0) | 8.3 (6.5-26.5) |
| Detection of MMCs | Undetectable | Undetectable in 9/9 samples | Undetectable in 6/7 samples |
| N-PC count (cells/μL) | 5.8 (1.4-32.0) | 17.2 (0.4-199.0) | 6.5 (0.9-26.8) |
| B-lymphocyte count (cells/μL) | 58.3 (11.3-324.9) | 1.8 (0.3-2.5) | 250.8 (18.2-511.5) |
| Peripheral blood | |||
| Patients with positive MRD the day before HDM | |||
| Number of patients evaluated | 17/18 | 14/18 | 10/18 |
| Peripheral blood cell count (106 cells/mL) | 4.7 (3.3-11.1) | 0.1 (0.1-0.6) | 5.2 (2.7-10.1) |
| Detection of MMCs | Detected in 12/18 samples | Detected in 6/14 samples | Detected in 0/10 samples |
| MMC count (cells/μL) | 0.1 (0-0.4) | 0.002 (0-0.2) | 0 |
| N-PC count (cells/μL) | 0.9 (0.1-4.2) | 0.9 (0.2-4.3) | 0.5 (0-8.5) |
| B-lymphocyte count (cells/μL) | 24.4 (3.1-94.4) | 0.2 (0.04-2.5) | 129.2 (40.5-389.3) |
| Patients with negative MRD the day before HDM | |||
| Number of patients evaluated | 9 | 9/9 | 5/9 |
| Peripheral blood cell count (106 cells/mL) | 4.6 (3.1-7.4) | 0.3 (0.1-1.6) | 4.0 (2.6-5.1) |
| Detection of MMCs | Undetectable in 9/9 samples | Undetectable in 9/9 samples | Undetectable in 5/5 samples |
| N-PC count (cells/μL) | 1.8 (0.5-3.4) | 3.6 (0.2-12.8) | 0.8 (0.03-3.8) |
| B-lymphocyte count (cells/μL) | 26.1 (7.3-443.7) | 0.3 (0.1-0.7) | 123.4 (76.4-265.0) |
Results expressed as median (range) ; Autologous stem cell transplantation (ASCT) ; High dose melphalan (HDM) ; Minimal residual disease (MRD) ; Normal plasma cell (N-PC) ; Multiple myeloma cell (MMC)