Table 3. Free energy differences (in kcal/mol) for the described transformations.

	Bound state	Unbound state (in solution)	∆∆G bind	Exp. a
MOP→HMP	16.7 ± 0.6	-17.9 ± 0.5	-1.2 ± 1.1	-0.4±0.3
HMP→MOP	-17.5 ± 0.6	18.3 ± 0.2	0.8 ± 0.8	0.4±0.3

Error estimates are included.

^a Calculated as $\mathrm{\Delta }\mathrm{\Delta }{G}_{\text{MOP}\to \text{HMP}}^{\text{exp}.}=\mathrm{\Delta }{G}_{\text{bind}}^{\text{HMP}(\text{exp}.)}-\mathrm{\Delta }{G}_{\text{bind}}^{\text{MOP}(\text{exp}.)}=RT\text{ln}\left[K_i^{\text{HMP}(\text{exp}.)}/K_i^{\text{MOP}(\text{exp}.)}\right].$